Classification:
Chemiluminescent reagents
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- Description
- Usage Introduction
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[English name] 3-Aminophthalhydrazide
[Chinese name] luminol / 3-aminobenzoylhydrazide / Luminol
【CAS#】521-31-3
[Molecular weight] 177.16
[Formula] C8H7N3O2
[Storage condition] room temperature, away from light and moisture
[Chemical properties] soluble in alkaline solution, soluble in dilute acid, almost insoluble in water, insoluble in alcohol. Neutral or light acid solutions show strong bright blue fluorescence when exposed to ultraviolet light.
[Application] chemiluminescent reagent, commonly used in chemiluminescent immunoassay, such as metal cations and blood detection.
[Luminescent rate detection] the optimal fluorescence wavelength is 425nm (detect the chemiluminescence rate in 60mMK2S2O8,100mK2CO3,pH11.5 solution).
Luminol is one of the most commonly used liquid-phase chemiluminescent reagents because of its simple structure, easy synthesis, good water solubility and high quantum efficiency. Since Albrecht first reported the chemiluminescent reaction of luminol and oxidant in alkaline solution in 1928, people have been very active in the study of the chemiluminescent system, which makes the chemiluminescence very active Optical systems are used in many fields.
Key words:- Fluorescent molecular
- Substrates
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