Parenzyme (EC 188.8.131.52) is a kind of protease. It is a serine proteolytic enzyme extracted from the pancreas of cattle, sheep and pigs. In vertebrates, it acts as a digestive enzyme. After trypsinogen, the precursor of trypsinase, is synthesized, secreted as a component of pancreatic juice, and decomposed into activated trypsinase restricted by enterokinase or trypsinase. It is an endonuclease that cuts off the carboxyl side of lysine and arginine residues in the polypeptide chain. It not only plays the role of digestive enzymes, but also restricts the decomposition of precursors of chymotrypsinogen, carboxypeptidase, phospholipase and other enzymes, and plays an active role. It is the most specific protease and becomes an indispensable tool in determining the amino acid arrangement of proteins.
Trypsinogen is composed of more than 220 amino acids with 6 pairs of disulfide bonds and its molecular weight is about 24 kD. The serine residues in the active site are indispensable serine proteases. Under the active self-catalysis of enterokinase, the peptide bond between N-terminal lysine and isoleucine residue of proenzyme was hydrolyzed, and the valerian-tian-tian-lys6 peptide was released to produce active trypsin. Trypsin can be used for cell digestion. In addition, trypsinase is one of the instrumental enzymes necessary for insulin production.
Formulation: dry powder, or liquid
Preservation buffer: 50mMHAc, 50mM CaCl2, 100mM Nacl, pH 3.5
Enzyme activity: 120 U/mg protein
Activity unit: 25 C, pH 8.1, reaction system 3.0ml (25px light path). Enzymatic hydrolysis of TAME per minute increased the absorption value at 247nm by 0.18, defined as a U unit.
Source: Gene recombinant expression
Enzymology number: EC184.108.40.206
Molecular weight: 24KDa (SDS-PAGE detection)
Purity: > 90% (SDS-PAGE test)
Preservation Conditions: Store at 4 for one year or so. (Depending on the dosage, it can be stored at - 20 or - 80 for a longer time, avoiding repeated freezing and thawing as far as possible)
Transportation Conditions: Low Temperature Ice Bag
Purpose: Used in the production of recombinant insulin
Safety Tips: Not Used in Human Experiments
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