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Analysis of Common Components and Principles of Magnetic Bead Extraction Reagents The magnetic bead method of nucleic acid purification technology uses nano-scale magnetic beads. The surface of the magnetic beads is labeled with a functional group that can react with nucleic acid by adsorption. Magnetic Silica Particle means that the surface of magnetic beads is wrapped with a layer of silicon material to adsorb nucleic acids. Its purification principle is based on the purification method of glass milk. So what are the common ingredients in the reagents using magnetic bead extraction, and what is the principle? The editor of Desheng lists some common ingredients in turn, SDS (sodium dodecyl sulfate)-properties:
Preparation of biological buffer TAPS solution The biological buffer TAPS is a biochemical reagent commonly used in biochemical experiments to maintain the pH of the reaction system, and is widely used in experiments on nucleic acid, DNA and RNA. Its full name is N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid CAS: 29915-38-6, and the buffer range is 7.7-9.1.
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What are the classifications of chemiluminescent reagents Chemiluminescence immunoassay (CLIA) was born in 1977 and is more advanced than many traditional detection methods. This technology has high sensitivity, wide linear range and wide application range. Moreover, compared with radioimmunoassay (RIA), fluorescence immunoassay (IFA) and enzyme-linked immunoassay (EIA), it is easy to operate, fast in response, no radioactive pollution, simple and stable instrument and can be automated.
2 days ago
Acridine ester as a weather vane for chemiluminescence marking Acridine esters are a class of chemical substances that can be used as chemiluminescent markers. In alkaline H2O2 solutions, when the molecules of acridine esters are attacked by hydrogen peroxide ions, the substituents on the acridine ring can match those on the acridine ring. The C-9 and H2O2 form unstable dioxyethane (this dioxyethane can quickly decompose into CO2 and electronically excited N-methylacridone, which emits photons when it returns to the ground state). Substituted acridine compounds can be used as chemiluminescent labels. Acridine ester compounds are a class of promising non-radioactive nucleic acid probe markers. They are used as luminescent probes of DNA. They have high luminescence quantum yield and good stability. The stability has no effect, and the chemiluminescence reaction can be carried out directly in alkaline medium. To
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Acridine ester chemiluminescence can study and analyze the DNA damage of environmental pollutants The rapid development of molecular biology and genetic engineering has provided a large amount of information for the study of human genes, and has brought revolutionary changes to the diagnosis of genetic diseases and the study of pathogenic mechanisms. Establish simple, rapid and sensitive detection of DNA hybridization and inheritance The method of toxic effect is a very meaningful research topic. For the detection of DNA damage by genotoxic substances, the commonly used method is to detect the different signals generated by the interaction between the indicator and the DNA before and after hybridization. Now more indicators are used. The agent is acridine ester.
Glutamate dehydrogenase is so important for tumor and cancer screening The CAS number of glutamate dehydrogenase (GDH) is 9029-12-3. It is a mitochondrial enzyme that is commonly found in animals, plants and microorganisms. It belongs to the superfamily of amino acid dehydrogenases. Play a key role in the metabolic process. Under normal circumstances, the enzyme uses NAD+ and/or NADP+ as coenzymes to catalyze the reversible oxidative deamination of L-glutamic acid to generate ɑ-ketoglutarate, thereby participating in the tricarboxylic acid cycle (TCA cycle), signal transduction, nitrogen and Regulation of carbon metabolism and other physiological activities. It plays an important role in energy regulation and maintaining cell homeostasis.
Lactate dehydrogenase indicators help you find the "invisible killer" Lactate dehydrogenase is a metalloprotein containing zinc ions. It is an isoenzyme that catalyzes the mutual conversion of lactic acid and acetone. It is a hydrogen transferase and one of the important enzymes for anaerobic glycolysis and gluconeogenesis. Lactate dehydrogenase It is a key enzyme that catalyzes phenylpyruvate to phenyllactic acid in microorganisms. It is also a vital oxidoreductase in the glycolysis pathway in organisms. It can reversibly catalyze the oxidation of lactic acid to pyruvate. The catalytic reaction is anaerobic The final product of glycolysis.
Problems to be noticed in clinical application of separation gel blood collection tube Separation gel blood collection tubes have been widely used in clinical laboratories. Separation gel can form an isolation layer between cell components and serum (plasma), effectively prevent the exchange of substances between blood cells and serum (plasma), and ensure serum (plasma) within a certain period of time. ) Stability of ingredients. There are generally two types of separation gel blood collection tubes commonly used in laboratories: serum separation gel coagulation tube and plasma separation gel anticoagulation tube.