9 biological buffers for cell culture

Release time:

2021-10-20


One of the most important properties of Good's Buffers is that they are not toxic to cells. Therefore, these chemicals are widely used in cell culture to keep the pH of the experiment under control. Due to their special functions, many of Good's buffers/biological buffers are considered ideal choices for cell separation, cell culture, enzyme assays and many other biochemical applications. Here are 9 biological buffers that you may consider in your research:

1) HEPES

1. HEPES can be used to buffer mammalian cell culture in the open system of high density culture and the closed system of high and low density culture;

2. Used as a buffer for in vitro fertilization and embryo culture.

2) MOPS

1. Many studies have reported its application in bacterial cell culture media (for example, Enterobacter 4, Lactococcus lactis 5 and Legionella pneumophila;

2. Cell culture medium for yeast and mammalian cells. Please note that only concentrations below 20 mM are suitable for mammalian cells to work;

3. Used in cell culture media to study the meiotic regulation of mouse oocytes.

3) MES

1. Some studies have reported its use in cell culture media for bacterial growth (such as halophilic bacteria);

2. Cell culture medium for yeast and mammalian cells. Please note that only concentrations below 20 mM are suitable for mammalian cells to work;

3. For plant culture medium (but the concentration is about 10 mM-it is toxic to most plants at higher concentrations);

4. Used in the culture medium to start the growth of pine and fir trees.

4) BES: Used for phage adsorption in the culture medium.

5) MOPSO: used as a buffer component of the charcoal yeast extract medium for the growth of Legionella pneumophila.

6) ACES

1. Used as a buffer component of the charcoal yeast extract medium for the growth of Legionella pneumophila;

2. Used in cell culture media for the hairy roots of Catharanthus roseus, a plant commonly known as Catharanthus roseus.

7) TAPS: used for dinoflagellate experiments in cell culture media (for example: marine plankton).

8) Bicine: used for the cultivation of ammonia fungi.

9) Tricine

1. Used in bacterial culture medium to prevent iron salt precipitation;

2. For animal tissue culture.

The above is the information of 9 kinds of biological buffers used in cell culture collected by Desheng, which can help you understand which buffers are more suitable for your application. As a professional manufacturer of biological buffers, Desheng has the ability to provide you with better quality Guaranteed products, welcome to inquire!