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New Trinder's reagent emerged as the times require in the development of medical diagnosis industry

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New Trinder's reagent emerged as the times require in the development of medical diagnosis industry

Classification:
Knowledge encyclopedia
Author:
2019/07/22 17:13

Introduction:
 
There are many principles for in vitro diagnostic reagents or kits. For example: Enzyme-Linked ImmunoSorbent Assay (ELISA), enzyme method, colloidal gold method, immunoblotting and so on.
 
Each principle can be applied to the detection of multiple items to be measured. For example, based on the principle of Trinder reaction (also known as enzymatic method), it can be used to detect uric acid, creatinine, blood sugar, cholesterol, triglyceride and so on in blood test.
 
Trinder's Reaction and Trinder's Reagent
 
Trinder's reaction:
 
This reaction was first proposed by Trinder in 1969, hence the name Trinder reaction. The principle is that hydrogen peroxide (H2O2) produced by the tested substance through enzymatic action in the presence of 4-aminotipyrine (4-AAP) and peroxidase (POD) can make the chromogenic substance produce red quinone imide compounds. Phenol was initially used as a chromogenic agent, and many compounds were substituted for phenol, which greatly improved the sensitivity of the reaction. Trinder reaction is the basis of enzymatic determination of glucose, cholesterol, triglyceride and uric acid.
 
 
 
Trinder's reagent:
 
In Trinder's reaction, the chromogenic substance or chromogenic agent is Trinder's reagent, also known as chromogenic substrates or hydrogen donors.
Types of Trinder's Reagents
 
Traditional Trinder's reagent:
 
Phenols: phenol, 4-chlorophenol or sodium 3,5-dichloro-2-hydroxybenzenesulfonate, etc.
 
Anilines: N, N-dialkyl aniline, N, N-dialkyl-m-toluidine, etc.
New Trinder's reagent:
 
Sodium aniline sulfonate (10 kinds): ADPS, TOOS, TOPS, ADOS, HDAOS, DAOS, ALPS, MAOS, MADB, TODB.
 
Relations among Enzymes, New Trinder's Reagents, Good's Buffers
1. Trinder reaction involves three steps:
 
Step 1: Mix the new Trinder's reagent, 4-AAP, the corresponding oxidase (glucose oxidase, uric acid oxidase, etc.) and the peroxidase.
 
In step 2, the Good's buffer is added to the mixture obtained in step 1.
 
Step 3, add the test (serum) to the first two steps to mix, then color reaction occurs, through the determination of absorbance, we can calculate the value of the item to be measured. For example, blood sugar in serum, uric acid value and so on.
 
2. Why use enzymes, because there are too many substances in serum, and enzymes have specificity, such as adding glucose oxidase, can only catalyze the oxidation of blood sugar to hydrogen peroxide, but not the oxidation of uric acid in serum.
 
3. Because of the use of enzymes, the pH value has a great influence on the activity of enzymes. Each enzyme has its own high activity PH range. For example, the optimum pH value of peroxidase is about 7.0, the optimum pH value of glucose oxidase is 3.5-6.5, and the optimum pH value of uric acid oxidase is about 8.5.
 
4. Different Good's buffers can provide suitable buffer intervals for different enzymes.