Sodium aniline sulfonate (10 kinds): ADPS, TOOS, TOPS, ADOS, HDAOS, DAOS, ALPS, MAOS, MADB, TODB.
1. Trinder reaction involves three steps:
Step 1: Mix the new Trinder's reagent, 4-AAP, the corresponding oxidase (glucose oxidase, uric acid oxidase, etc.) and the peroxidase.
In step 2, the Good's buffer is added to the mixture obtained in step 1.
Step 3, add the test (serum) to the first two steps to mix, then color reaction occurs, through the determination of absorbance, we can calculate the value of the item to be measured. For example, blood sugar in serum, uric acid value and so on.
2. Why use enzymes, because there are too many substances in serum, and enzymes have specificity, such as adding glucose oxidase, can only catalyze the oxidation of blood sugar to hydrogen peroxide, but not the oxidation of uric acid in serum.
3. Because of the use of enzymes, the pH value has a great influence on the activity of enzymes. Each enzyme has its own high activity PH range. For example, the optimum pH value of peroxidase is about 7.0, the optimum pH value of glucose oxidase is 3.5-6.5, and the optimum pH value of uric acid oxidase is about 8.5.
4. Different Good's buffers can provide suitable buffer intervals for different enzymes.