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Synthesis process of luminescent substrate acridine salt DMAE-NHS
Acridine salt DMAE-NHS is a member of acridine ester compounds. Acridine ester compounds are a very effective chemiluminescent labeling substance, which has strong and stable luminescence, few interference factors and high signal-to-noise ratio. , And the requirements for the instrument are relatively simple, and the detection sensitivity and precision are high, which is conducive to the realization of fully automated operation and plays an important role in chemiluminescence immunoassay.
Acridine ester is a general term for acridine salts, which refers to a class of chemical substances that can be used as chemiluminescent markers. There are many different series of acridine esters, including acridine salt DMAE-NHS, acridine ester NSP-DMAE- NHS, acridine salt NSP-SA, acridinium ester NSP-SA-NHS, acridinium ester NSP-SA-ADH, acridinium ester ME-DMAE-NHS, etc. Next, we will introduce the synthesis method of acridine salt DMAE-NHS .
According to published reports, the most extensive process for preparing acridine salt DMAE-NHS is summarized as a seven-step reaction method. In view of the complex reaction steps of this method, the high cost of raw materials and reagents, and the disadvantages of large-scale preparation, many researchers optimized the traditional seven-step method based on the original literature and developed a five-step chemical reaction to prepare acridine. A new method of pyridine salt. They use 3,5-dimethyl-4-hydroxybenzoic acid and acridine 9 carboxylic acid as starting materials, and undergo a 5-step synthesis reaction of esterification, condensation, hydrolysis, succinimidation and methylation, and finally obtain The target product, acridine salt, DMAE-NHS, has a purity of more than 98%.
The method has few synthesis steps, simple reaction operation, easy implementation, eliminating the use of expensive reagents and hydrogen bromide acetic acid solution in the original route, and the final product does not need column chromatography purification, which saves costs and reduces environmental impact. Pollution is convenient for large-scale preparation.
The acridinium ester luminescence system has obvious advantages and has better sensitivity in in vitro diagnosis. At present, the application of acridinium ester chemiluminescence immunoassay technology has gradually developed into a trend in the fields of medical testing, diagnostic analysis, environmental testing, and food testing. . In addition to the above 6 types of acridinium products, Desheng can also provide reliable quality luminol and isoluminol products, which can meet the scientific research and production needs of scientific research institutions and kit manufacturers.