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Biological buffer TAPS for capillary zone electrophoresis

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Biological buffer TAPS for capillary zone electrophoresis

Classification:
News
Author:
2020/10/05 16:00

The biological buffer TAPS can be used as a buffer for nucleic acid DNA or RNA in capillary zone electrophoresis. Capillary zone electrophoresis is different from electrophoresis in electric pools. Electrophoresis in electric pools usually requires agarose gel or other electrophoresis gels. The effect of separating DNA or nucleic acid, and various particles can be separated directly in the capillary.

The capillary used for electrophoresis is an elastic quartz capillary with an inner diameter of less than 100 μm (usually 50 to 75 μm) and a length of generally 30 to 100 cm to analyze charged solutes. In order to reduce electroosmotic flow and adsorption, the inner wall of the capillary can be coated.

Capillary zone electrophoresis is an electrophoretic separation and analysis method that uses elastic quartz capillary as the separation channel, uses high-voltage direct current electric field as the driving force, and realizes separation based on the difference in mobility and distribution behavior of the components in the sample.

Biological buffer TAPS reagent powder

The quartz capillary column used for capillary electrophoresis has a negative charge on its inner surface when the pH value is greater than 3, and forms an electric double layer when it comes in contact with the buffer. Under the action of a high-voltage electric field, the buffer on the side of the electric double layer is formed due to It is positively charged and moves in the negative direction, thereby forming an electroosmotic flow.

At the same time, in the buffer solution, the charged particles move in the opposite direction of the charged polarity at different speeds under the action of the electric field to form electrophoresis.

The migration speed of charged particles in the capillary buffer is equal to the vector sum of electrophoresis and electroosmotic flow. Various particles are separated due to different migration speeds caused by different factors such as the amount of charge, mass, volume, and shape.

You can also fill the HPLC stationary phase into the capillary or coat the stationary phase on the inner wall of the capillary, and use electroosmotic flow as the driving force of the mobile phase to form capillary electrochromatography. This mode has both electrophoresis and liquid phase. Chromatographic separation mechanism.

In capillary electrophoresis, the tube needs to be filled with buffer to ensure the operation, organic solvent modification and surfactant can be added, and the running buffer needs to be degassed before use. TAPS buffer is very suitable for nucleic acid experiments, so it is very suitable to use TAPS buffer for DNA or RNA capillary zone electrophoresis buffer.

The reagent for preparing TAPS buffer is synthesized by tris THAM and 1,3 propane sultone in alcohol solvent. Desheng also produces THAM buffer, so it has technical and cost advantages in the synthesis of TAPS. It is a recommended biological buffer manufacturer.