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How to do blood analysis after blood collection tube
Before blood collection with a vacuum blood collection tube, various reagents such as separating gel, coagulant, heparin, and dipotassium EDTA will be added to deal with various blood tests, but this is only the first step in blood testing, which is a blood sample A pre-processing process. After the blood collection tube collects blood, how is the follow-up blood analysis done?
The current blood analysis is done in the hematology analyzer. In the past, the manual method of microscope counting was used. Due to the random error of the operation process, the system error of the experimental equipment and the inherent error of the detection method, the experimental result of the microscope method of cell counting The accuracy is greatly affected.
With the development of medicine and the application of high-tech technology, especially the application of computers, the application of blood cell analyzers has made remarkable progress. Now it can detect more than a dozen to dozens of parameters at the same time. Now my country has basically popularized blood cell analyzers, which has significantly improved the quality of blood cell analysis. However, the excessively mechanical working method reduces the requirements for professional knowledge, and some problems such as excessive trust in the results of the instrument have appeared, which requires us to pay more attention.
Blood testing-the top priority of in vitro diagnostics
1. Sample collection and use of blood anticoagulant
Specimen collection and the use of anticoagulants are key issues in quality control before the use of blood cell analyzers, which have a great impact on the results, but people often do not pay attention. If the blood collection is not smooth or the anticoagulation effect is poor, the platelet count often decreases; if the blood collection is not smooth, the presence of small clots that are not easy to detect with the naked eye can cause the counting hole of the blood cell analyzer to be blocked and result in error. The most suitable anticoagulant for blood cell analysis is EDTA salt, which has little effect on blood cell morphology and platelet count. It is recommended to use EDTA-K2 as an anticoagulant for blood cell analysis. At the same time, it should be noted that EDTA-dependent platelet aggregation can lead to pseudothrombocytopenia, which is mainly seen in tumors, autoimmune diseases, pulmonary heart disease, late pregnancy, liver disease, toxemia and some unexplained diseases. Count platelets by diluent and other methods. To
2. To rely too much on the results of instrumental analysis, reduce the requirements for professional knowledge, and reduce the ability to understand cell morphology. So far, any blood cell analyzer can only be a screening method for blood cell classification. In the presence of immature cells, the analysis results are not reliable, and manual microscopic review is necessary.
3. Comparability of blood cell analysis results
The difference between the detection results of the same item of the same specimen by the detection system of different models and reagents is sometimes far greater than the acceptable error range. There are multiple blood cell analyzers from the same or different manufacturers in hospitals.
To sum up, while using the high efficiency and convenience brought by the blood analyzer, we also need to pay attention to the defects of the instrument itself, not to be overly dependent, and to continuously learn and improve professional knowledge. Desheng has more than ten years of experience in the field of blood pretreatment, and has significant advantages in blood collection tube additives, serum separation gel, coagulant, heparin, and EDTA anticoagulant.