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The determination method and clinical significance of serum IgD and IgE
The content of IgD in normal human serum is very low, accounting for only 0.2% of the total amount of serum immunoglobulin. IgD can be produced at any time during individual development, with a half life of 2.8 days. The biological function of serum IgD is still unclear. Membrane-bound IgD (mIgD) constitutes BCR and is a sign of B cell differentiation and maturity. Immature B cells only express mIgM, and mature B cells can express both mIgM and mIgD. Naive B cell (naive B cell); mIgD on the surface of activated B cell or memory B cell gradually disappears.
IgE is the immunoglobulin with the least content in normal human serum, and it is secreted by plasma cells in submucosal lymphoid tissues. Its important feature is that the sugar content is as high as 12%. IgE is a cytophilic antibody that can cause type I hypersensitivity reactions. IgE may be related to the body's anti-parasitic immunity.
The measurement of IgD and its clinical significance
Measurement methods: IgD measurement methods include radioimmunoassay, latex particle immunoturbidimetric analysis, and enzyme-linked immunosorbent assay. The most commonly used clinically is the enzyme-linked immunosorbent assay to determine IgD.
Clinical significance: Elevated serum IgD is mainly seen in late pregnancy, IgD myeloma, thyroiditis and heavy smokers. IgD reduction is seen in primary agammaglobulinemia, silicosis (silicosis), and after treatment with cytotoxic drugs.
The measurement of IgE and its clinical significance
Determination methods: The determination methods of total IgE include chemiluminescence immunoassay, radioimmunoassay, latex particle immunoturbidimetry and enzyme-linked immunosorbent assay. Among them, acridine esters are the most widely used reagents in chemiluminescence analysis. It is more common to determine total IgE in clinical practice. There are many types of acridinium esters, mainly. Specific IgE detection methods: mainly include immunospot method and enzyme-linked immunosorbent assay. Commercial kits provide a variety of specific allergens, including food, inhalation, and drug allergens. With these specific allergens, specific IgE antibodies in serum can be detected.
Clinical significance: 1. Type I allergic diseases, such as idiopathic asthma, idiopathic dermatitis, allergic rhinitis and other IgE often increase. 2. Non-allergic diseases, such as IgE myeloma, parasitic infections and other elevated IgE. 3. Acute or chronic hepatitis, SLE, rheumatoid arthritis and other diseases can increase IgE. 4. IgE reduction is seen in primary agammaglobulinemia, tumors and after the application of chemotherapy drugs.
In general, the determination of serum IgD and IgE is of great significance for clinical diagnosis. The proteins in the blood include not only IgG, IgM, but also hemoglobin and albumin. Desheng is a professional manufacturer of blood test related reagents. The products include Blood collection tube additives, enzyme preparations and antibodies, etc.