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Instructions for use of biological buffer TAPS (CAS29915-38-6)
TAPS is a commonly used biological buffer. It is usually synthesized from Tris and 1,3-propane sultone. The buffer range is 7.7-9.1. It becomes a buffer system composed of weakly basic Tris and propanesulfonic acid without adding hydrochloric acid or other acids. This article describes how to use it.
The relative molecular mass of TAPS can be calculated by the molecular formula C7H17NO6S or directly inquired to be 243.28. According to the required amount and molar concentration of the solution, accurately calculate the mass to be weighed and accurately weigh it with an analytical balance. Also pay attention to the purity of the purchased reagents, correct the weighing error to avoid insufficient weighing due to low content.
Biological buffer TAPS powder
TAPS dissolution and volume:
Dissolve the weighed TAPS reagent powder completely in deionized water. Be careful not to add too much water when dissolving, ensure that all are dissolved, and finally continue to add water to make the volume. Pay attention to the temperature when setting the volume. If the temperature changes too much due to exothermic or endothermic heat during dissolution, the volume should be fixed when the temperature is close to room temperature. After configuration, the volumetric flask is sealed with a lid, which can be kept ready for use at any time, the TAPS solution A is marked, and the concentration configuration date and other information can be marked.
NaOH lye configuration:
As with TAPS, accurately weigh the quality of NaOH according to the required amount, then dissolve it in deionized water, and finally make a volume at room temperature and mark it as Solution B.
TAPS buffer configuration:
According to the required buffer pH value, the above solution A and solution B are mixed in a certain ratio to obtain the desired TAPS buffer solution. If a buffer solution with a specific pH value is needed, the approximate concentration buffer solution can be prepared in proportion, and then the specific pH value can be measured by a pH meter to adjust the ratio of solutions A and B to adjust to the desired pH value. Note that the temperature is controlled at room temperature. When NaOH cannot be used, KOH can be used instead.
pH 4.6 1,000ml(A)+0ml(B) (A):(B)=5:0
pH 7.8 1,000ml(A)+200ml(B) (A):(B)=5:1
pH 8.3 1,000ml(A)+400ml(B) (A):(B)=5:2
pH 8.6 1,000ml(A)+600ml(B) (A):(B)=5:3
PH 9.0 1,000ml(A)+800ml(B) (A):(B)=5:4
In addition to the conventional TAPS buffer, there are some others, such as 10X Tris-TAPS-EDTA buffer, the composition contains 500 mM Tris; 500 mM TAPS; 10 mMEDTA, TAPS can be used with Tris, then Tris does not need to add other Acid (hydrochloric acid, acetic acid, phosphoric acid, etc.), TAPS itself is equivalent to Tris + propanesulfonic acid.
TAPS buffer preparation is relatively simple. You can buy the reagent powder directly, and then prepare your own buffer solution is much more convenient than going directly to buy. Direct preparation is faster than express delivery in urgent use, and it saves money. And also avoid waste. Desheng has special buffers such as Tris and TAPS, and can also provide configured emergency buffers.